MyScope
training for advanced research

Search


Glossary terms about Autofluorescence

Autofluorescence
Fluorescence derived from naturally occurring biomolecules within a specimen.


4 pages mention Autofluorescence

Averaging
Show 0 2 4 8 16 averages var graphBottom, graphTop, scanBottom, scanTop, btnSelected, doAverage; graphBottom = $('#graphBottom'); graphTop = $('#graphTop'); scanBottom = $('#scanBottom'); scanTop = $('#scanTop'); btnSelected = $('#avg0'); $('#avg0').click(function() { doAverage(0, $(this)); }); $('#avg2').click(function() { doAverage(1, $(this)); }); $('#avg4').click(function() { doAverage(2, $(this)); }); $('#avg8').click(function() { doAverage(3, $(this)); }); $('#avg16').click(function() { doAverage(4, $(this)); }); doAverage = function(num, obj) { //btnSelected.removeAttr('disabled'); //obj.attr('disabled','disabled'); btnSelected = obj; graphBottom.css('background-position-x', graphTop.css('background-position-x')); graphTop.css('background-position-x', '-' + 398 * num + 'px'); scanBottom.css('background-position-x', scanTop.css('background-position-x')); scanTop.css('background-position-x', '-' + 400 * num + 'px'); scanTop.css('width', '0'); scanTop.stop().animate({width: 400}, 1000); graphTop.css('width', '0'); graphTop.stop().animate({ width: 398}, 1000); } Images of a pollen grain autofluorescence collected using identical gain values but using different line averaging values. 
Collecting Z stacks
(As an aside, this data was derived by collecting the autofluorescence signal emitted predominantly by the chitin exoskeleton when excitated with 488nm (blue) laser light. 
Fluorescence microscopy
Some cell components (such as chlorophyll and phenolic compounds) are inherently fluorescent (autofluorescence). 
Practical image acquisition
Comparisons like this also allow a very objective means to evaluate labelling protocols when titrating antibodies. Many samples will show some level of autofluorescence