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Glossary terms about Epifluorescence

Fluorescence resulting from epi-illumination, where the excitation light and emission light are on the same side of the specimen. A dichroic mirror is required for epi-illumination.

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Practical image acquisition
If the pinhole is opened to its maximum value, the confocal microscope can produce images that are similar to those produced by a widefield, epifluorescence microscope.