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Glossary terms about Refractive Index Mismatch

Refractive Index Mismatch
This occurs where the specimen is mounted in such a way that the optical properties (usually refractive index or coverslip thickness) of the mounted sample are not in compliance with the design requirements of the objective. This can result in severe spherical and/or chromatic aberration in the image and is one of the most common problems in confocal microscopy.

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