training for advanced research


Glossary terms about Immersion Oil

Immersion Oil
A synthetic immersion liquid specified as suitable for use in the space between the front of the immersion objective and the glass coverslip. This immersion liquid is considered in computing lens corrections and its refractive index and dispersion are critical.

4 pages mention Immersion Oil

Collecting Z stacks
Components of the confocal microscope
So if our specimen is in a permanent mounting medium of refractive index (n) ~ 1.5, we put immersion oil of the same index between the coverslip and the lens. 
Scanning and resolution