- Choose a low magnification to start with and ensure your contrast and brightness are adjusted so you can see an image. Adjust the scan speed to TV rate for easy viewing.
- It may be necessary to adjust the probe size (spot size) to match the magnification. Choose a large spot size for low magnification and a small one for high magnification.
- Focus the image. There may be a course and a fine focus control.
- Correct the stigmators (x and y) if the image is excessively streaky or blurry and focus again.
- You may be required to align the machine at this stage. If so there will be detailed instructions supplied to you. This might include changing an aperture to a larger or smaller size, adjusting the gun controls to provide the brightest image, and adjusting the “wobbler” to align the beam axis.
- Once the machine is ready to use, move your sample about with the X and Y translators until you are looking at a region of the sample you want to see in more detail. You may want to rotate or tilt the sample to get the best angle of view. Check that you have enough room around your sample before tilting so as not to bump into anything in the chamber. Sometimes you will need to change the height (z-axis) of your sample (lower for greater depth of focus or to allow tilting; higher for greater resolution)
- Increase your magnification.
- Adjust the brightness and contrast again for a comfortable view.
- Now adjust your focus and correct the stigmators until the image is crisp and in focus.